There needs to be “a broader, long-term strategy” that aims not only to eradicate poverty but to build infrastructure such as roads and ports and to develop “global public goods” by protecting air and water quality and fighting communicable diseases, Mr. Annan said in an address delivered on his behalf by José Antonio Ocampo, the Under-Secretary-General for Economic and Social Affairs, to the Ministerial Forum on Financing for Development in Paris.The eight MDGs, adopted at a UN summit in 2000, include the halving of extreme poverty, achieving universal primary education, improving maternal health and reducing child mortality.Mr. Annan said that achieving the MDGs on schedule is unlikely, however; it will require “at least a doubling of recent levels of official development assistance,” and that does not seem likely to happen soon.”Official development assistance has increased recently, but many developed countries currently face fiscal constraints. Total resources are still falling far short of needs,” he said.Calling on participants at the Forum to think creatively about what the public and private sectors can do to support development in poorer States, he hailed the International Finance Facility proposal of Gordon Brown, the United Kingdom’s Chancellor of the Exchequer, describing it as “one of the most innovative ideas.”
“I condemn the murder of Prahlad Goala. In view of the essential role of the media in maintaining democracy and good governance, this killing must be considered a heinous crime against society as a whole,” UNESCO Director-General Koïchiro Matsuura said in a statement.The Paris-based organization said Mr. Goala’s murder had followed the publication of a series of articles in which he linked local forestry officials to timber smuggling. He had received death threats after the articles were published in the Assamese-language daily Asomiya Khabar, according to the Committee to Protect Journalists (CPJ).The CPJ reported that 32-year old Mr. Goala was riding a motorcycle near his home in eastern Assam on 6 January when he was apparently rammed by a truck. When police arrived at the scene, in the district of Golaghat, they found that Mr. Goala had been stabbed several times.Today’s condemnation by the UNESCO chief is the latest in a long series he has issued recently over attacks on journalists around the world. UNESCO’s mandate includes the defence of freedom of expression and press freedom.
Hopes of economic progress fade without Harper at table, premiers say by Michael Tutton, The Canadian Press Posted Nov 22, 2012 7:03 pm MDT AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to RedditRedditShare to 電子郵件Email HALIFAX – Stephen Harper’s refusal to meet with the premiers dampens the chances of economic progress for provinces struggling with tepid growth and rising debt, several of the provincial leaders said ahead of Friday’s discussion in Halifax.Robert Ghiz, the premier of Prince Edward Island, said he would have welcomed the prime minister’s ideas at the table on how to improve the country’s “sluggish” economic growth.“There is a difference when he is here,” Ghiz said late Thursday after arriving in the port city.“Whether it’s skills development, university funding … immigration, trade, all these things. There are federal ties in all those areas.”Nova Scotia Premier Darrell Dexter, who is hosting the meeting, said it’s challenging for provinces to devise their spending plans without the prime minister’s input.“It’s difficult for us to know how we can best blend our own planning in with the funding of the federal government if we don’t know what their plans are,” Dexter said.The idea for such a gathering came up during the last premiers’ meeting in July, when they agreed to discuss how they could protect their provinces from a global economy in flux. That was before revised figures showing a higher federal deficit and growing concerns over the possibility of the U.S. economy going over its so-called fiscal cliff at the end of next month.The premiers invited Harper but he declined. His office has said he has met regularly with the premiers and the economy remains his prime concern.Harper’s absence from the meeting has also become fodder for opposition parties in the House of Commons this week, where they accused him of shirking his responsibilities.“Mr. Speaker, precisely what Canadians want from their leaders is not more meetings,” Harper said in response Thursday. “They want action from this particular government.”Still, that kind of response has not sat well with some, including Ontario Premier Dalton McGuinty.“I think we’re all disappointed that we couldn’t get an opportunity to meet with the prime minister and to build a strong plan for economic growth together,” McGuinty said earlier this week.“We’ll see what we can do by way of a concerted effort.”Quebec Premier Pauline Marois also criticized Harper for not attending, saying she couldn’t understand why he wouldn’t attend given his interest on the economy.Late Thursday, Marois and Alberta Premier Alison Redford had a separate meeting to discuss the possibility of shipping oil from the West. They agreed to explore the economic opportunities and possible environmental effects of the project.Glen Hodgson, chief economist at the Conference Board of Canada, said the premiers should avoid the divisions that have defined some of their past meetings and attempt to make progress on matters under their control, such as boosting immigration and knocking down interprovincial trade barriers.“What are provincial governments doing to deepen the skills of their workforce or to encourage more immigrants to work into the Canadian workplace and get rid of barriers between provinces?” he said.One highlight of the meeting is a presentation by Bank of Canada governor Mark Carney, who will discuss the bank’s outlook for the global and Canadian economies as well as national inflation.Carney has said the U.S. fiscal cliff is the most imminent threat facing the Canadian economy, and he has also repeatedly warned that personal debt levels have reached record highs, posing a risk to the economy if consumers can’t afford to carry their debt when interest rates rise.Manitoba Premier Greg Selinger said he’ll be listening carefully to any guidance Carney can provide as he reviews his province’s finances.“We’ll be looking to him for what his specific ideas are,” Selinger said.The meeting comes a week after federal Finance Minister Jim Flaherty released the government’s revised deficit projections that show a deficit of $26 billion — up $5 billion from the March budget forecast — as a result of global economic weakness carving into commodity prices and tax revenues.The gloomy numbers have also prompted Ottawa to delay its hopes of balancing the books until 2016-2017, a year later than previously forecast. Despite that, Flaherty said he would not touch fiscal transfers to the provinces.Dexter said one issue he would like to address is the $8.8-billion Building Canada Fund. The federal infrastructure program expires in 2014.“We’re given to believe there will be a new infrastructure program, but we’re not told what it is,” he said.In an email, Harper spokesman Andrew MacDougall said Infrastructure Minister Denis Lebel has been going through a six-month, national consultation on the fund.
Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window)Related”Retrograde and intolerable” – GPA concerned about Gov’t stance on State mediaSeptember 1, 2015In “Politics”Prime Minister’s Office to approve all headlines in Chronicle NewspaperAugust 26, 2015In “Politics”Acts of domestic terrorism must be condemned – PM NagamootooJune 8, 2016In “Crime” Prime Minister Moses Nagamootoo today (Monday), at the conclusion of the National Security Meeting at Camp Ayanganna, urged journalists to report accurate and factual information relating to the current situation in the country.Prime Minister Moses Nagamootoo “People need the information. It is a moment of anxiety and before you (media operatives) allow the rumours to take root, you need to fill the space with accurate and factual reports. It is very important in situations like this,” the Prime Minister was quoted in a government release as saying.Additionally, Prime Minister Nagamootoo commended government media operatives, particularly for the coverage and information that was provided last evening to the public as events unfolded at the Georgetown Prison, the release added.
File photo Image: Sasko Lazarov/RollingNews.ie Feb 25th 2019, 8:53 PM By Ruaidhrí Giblin 52,472 Views Image: Sasko Lazarov/RollingNews.ie Woman who made landlords’ lives ‘utter misery’ has five-year sentence reduced Rosemary Jones was previously given a two-year sentence for forgery and a consecutive three-year sentence for criminal damage. https://jrnl.ie/4512904 File photo 24 Comments Short URL A WOMAN WHO made her landlords’ lives an “utter misery” has had her five-year sentence for forgery and criminal damage cut to two years by the Court of Appeal.Rosemary Jones (49), with an address at Ballygraigue, Nenagh, Co Tipperary, pleaded guilty to forgery and criminal damage to the property of her landlords, the Egans, in Nenagh, after difficulties developed in the “landlord – tenant relationship” in 2014.She was given a two-year sentence for forgery and a consecutive three-year sentence for criminal damage by Judge Thomas Teehan at Nenagh Circuit Criminal Court on 15 March 2018.The sentencing judge had indicated a willingness to suspend the final year of Jones’ sentence if she was prepared to enter into a bond on certain terms but Jones was “not so prepared”, the Court of Appeal heard.She successfully appealed the severity of her sentence today and was accordingly resentenced to two years imprisonment with the final six months suspendedGiving judgment, President of the Court of Appeal Mr Justice George Birmingham said Jones had rented a three-bedroom house in Nenagh from the injured party in 2010.In 2014, he said difficulties developed in the landlord-tenant relationship. Jones had not paid any rent “for a very prolonged period”.She had been in receipt of Rent Supplement and the stage was reached where the landlord declined to sign forms with a view to securing vacant possession, the judge said. He said the forgery related to a signature on a Rent Supplement Review Form, although it didn’t appear she achieved any financial benefit from this.He said criminal damage was caused to kitchen furniture, a door, household property, a shower, walls and skirting boards.€10,000 damage Mr Justice Birmingham said Jones’ initial “reluctance” to plead guilty to criminal damage was on the basis that she wasn’t prepared to accept she deliberately set out to damage property.He said the landlords estimated the cost of repairing the damage to be somewhere in the region of €10,000. They had since acquired possession of the property and had sold it on at a loss. There was no doubt, he said, that the price achieved reflected the condition the premises was in.Mr Justice Birmingham said the sentencing judge clearly took a “very serious view” of the case. The sentencing judge may well have been influenced by the victim impact report prepared by Jones’ former landlords. It’s clear they were “tormented” by Jones and their lives had been made an “utter misery”, the judge said.Overall, he said the aggregate sentence of five years’ imprisonment was excessive and required intervention by the Court of Appeal.Furthermore, he said concurrent sentences, rather than consecutive sentences, given the close link between the separate offences, would not have been inappropriate.Mr Justice Birmingham, who sat with Mr Justice Patrick McCarthy and Ms Justice Isobel Kennedy, resentenced Jones to two years’ imprisonment with the final six months suspended. She was given a concurrent 12 month sentence for forgery.Jones was required to enter into a good behaviour bond for the suspended period and for two years post release. She undertook to be so bound. Monday 25 Feb 2019, 8:53 PM Share65 Tweet Email Tweet thisShare on FacebookEmail this article
Florida authorities arrested a driver after he was found with a live baby alligator and drugs during a traffic stop on July 4.Hardee County deputies stopped Anthony Richardson in the vicinity of State Road 64 and Pioneer Park in Zolfo Springs, Florida, on Thursday, ABC Action News reported. In addition to discovering illegal drugs on Richardson, officials spotted the alligator, which had its mouth taped shut, in a bag stashed inside his car. Before deputies searched the vehicle, Richardson said that he had the alligator in the front seat. Richardson also told deputies that he didn’t possess an alligator hunting/trapping license, but he received the alligator from a friend. Deputies said he had planned to release the creature into a nearby river.Deputies at the scene contacted the Florida Fish and Wildlife Conservation Commission (FFWCC) and safely released the reptile into the Peace River after discussing the incident with an FFWCC officer.According to deputies, Richardson was booked into the Hardee County Jail for multiple drug charges and illegally capturing or possessing an alligator.This isn’t the first time a Florida driver was found with a live alligator in their vehicle: In May, a woman pulled a live, 12-foot alligator from her yoga pants during a routine traffic stop in Florida. The woman, who was initially stopped for running a nearby stop sign, surrendered the gator to police following the incident.More on Geek.com:Woman Pulls Live Alligator From Yoga Pants During Traffic Stop in Florida Alligator Seen ‘Dragging Body’ Into Lake in Florida Man’s Hand, Foot Discovered in Florida Alligator’s Stomach Stay on target Georgia Man Catches Gigantic 14-Foot AlligatorHunters Catch ‘Monster’ 13-Foot Alligator in Mississippi River
Facebook Twitter Google+LinkedInPinterestWhatsApp#Bahamas, December 4, 2017 – Grand Bahama – Jobs are coming for Bahamians, particularly those resident in Grand Bahama now that a progressive three year deal is struck between Bahamas Paradise Cruise Line and the Bahamas Government. The Grand Celebration, which replaced the Bahamas Celebration will resume sailing from the Port of Palm Beach on December 24 and all new, and beginning in April is the Grand Classica which sets sail to Freeport in April.The contract was inked at the Manor House of the Grand Lucayan resort right after Thanksgiving between Prime Minister Hubert Minnis and CEO of Bahamas Paradise Cruise Line Oneil Khosa. Minister for Grand Bahama, Senator Kwasi Thompson said the contract calls for three conditions including that Bahamian businesses and products be used by the Celebration, that Bahamians be employed there and that with the second service in April, Bahamians be trained for job opportunities. Get ready was advice from Thompson for Bahamians.The prime minister said the contract signing is an indication of BPCL’s confidence and commitment to Grand Bahama. “They are obviously showing great support in Grand Bahama, and in the economy going to the extent of launching another vessel in April – that shows great confidence in our Bahamas,” he said.Cruises on board the Grand Celebration are from $149 from Palm Beach and the nine passenger deck cruise liner has a passenger capacity of 1900 people.#MagneticMediaNews#CruiseshipsreturntoGrandBahama Facebook Twitter Google+LinkedInPinterestWhatsApp Related Items:#CruiseshipsreturntoGrandBahama, #magneticmedianews
MIRAMAR, FLA. (WSVN) – Police are searching for the driver responsible for a hit-and-run crash in Miramar that left a man dead.According to investigators, the crash happened just after 2 a.m. on Sunday, along the 9500 block of Miramar Parkway.Police said the victim, identified as 26-year-old Antonio Gort, was traveling at a high rate of speed on the eastbound lanes when he was sideswiped by another vehicle, causing him to veer left into the median and crash into a tree.The impact caused Gort’s black 2015 Audi sedan to catch fire.The driver of the second vehicle fled the scene on foot before anyone could get there.An investigation has now been opened and police are searching for the driver.If you have any information on this hit and run, call Broward County Crime Stoppers at 954-493-TIPS. Remember, you can always remain anonymous, and you may be eligible for a $3,000 reward. Copyright 2019 Sunbeam Television Corp. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
OPA-LOCKA, FLA. (WSVN) – Rescue crews took three people to the hospital after they were involved in a hit-and-run rollover wreck in Opa-locka.Miami-Dade Police and Fire Rescue units responded to the scene of the crash near Northwest 22nd Avenue and 135th Street, Sunday morning.Officials said one of the cars was completely on its back and another was on its side against a power pole.Rescuers used the jaws of life to remove two victims who were trapped in the vehicle that had struck the power pole.“It was kind of a difficult scenario, being that the vehicle was on its side, and the victims were trapped on top of each other,” said MDFR Capt. Christopher Morales. “One of them had an extremity that was kind of pinned by the roof of the vehicle, so initially we had to stabilize the vehicle in order to gain access, just to do patient care.”Paramedics transported the three victims to an area hospital, where they are listed in stable condition.Police are searching for the driver of the car that caused the collision. They believe alcohol played a role. Copyright 2019 Sunbeam Television Corp. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
Facebook0TwitterEmailPrintFriendly分享The KSRM News Department compiled some of the top stories from this past week. Thursday 11/8Audio PlayerThursday-1108.mp3VmThursday-1108.mp300:00RPdPCHS Seeks Solution For Psychiatric Patients On The Kenai Peninsula, Revenue From Personal Use Dipnet Fishery Down 35%, Always Be ‘Winter Ready’, Field Of Flowers Signage Approval Moved To Next Meeting Monday 11/5Audio PlayerMonday-1105.mp3VmMonday-1105.mp300:00RPdKenai To Install Security Cameras At Police, Fire Department, State Making Major Progress Addressing Untested Sexual Assault Kits, Governor Walker: LNG Project “Brightest Spot On Economic Horizon”, Troopers Report Few Arrests Over Halloween Weekend Wednesday 11/7Audio PlayerWednesday-1107.mp3VmWednesday-1107.mp300:00RPdUnofficial Results: Micciche, Carpenter Win General Election, Dunleavy Maintains Lead In Race For Governor, Stand For Salmon Defeated In General Election, Young Secures Victory For Alaska’s Sole Congressional Seat Tuesday 11/6Audio PlayerTuesday-1106.mp3VmTuesday-1106.mp300:00RPdRunner Completes His Journey From The Kenai Peninsula To Key West, Florida, Officials Hope To Certify Election Results By November 23, DOC Reports Ninth Inmate Death This Year, ADOT&PF Reminds Drivers To Be Avalanche Aware Friday 11/9Audio PlayerFriday-1109.mp3VmFriday-1109.mp300:00RPdLNG Project Schedule Calls For Work To Start In 2020, Sen. Sullivan “Kenai Peninsula Is A Bright Spot In The State”, NPR-A Lease Sale Smaller Than Last Year’s, Veterans Day Ceremony At Soldotna Sports Complex
SEE RELATED: Ziff Says Goodbye to PrintA little more than a month after discontinuing the print edition of PC Magazine and taking the brand online-only, publisher Ziff Davis Media has sold its 1UP Digital Network to Hearst’s Ugo Entertainment. Financial terms were not disclosed.In conjunction with the sale, Ziff said it has shuttered Electronic Gaming Magazine. The January issue was its last. It was not immediately clear if EGM will continue online or if any layoffs were associated with the deal. A Ziff Davis spokesperson did not immediately return requests for comment.According to CEO Jason Young, the actions help Ziff Davis “pay down debt and shift our full focus to our core PCMag Digital Network Business.” In June, a New York confirmed Ziff’s reorganization plan, allowing the company to emerge from bankruptcy protection which it filed for in March.In addition to 1UP.com, the network includes gaming sites MyCheats.com, GameVideos.com and GameTab.com. GCA Savvian Advisors acted as Ziff’s financial advisor in the transaction. Hearst acquired the Ugo Networks, an online entertainment site targeting men aged 18 to 34, in July 2007. It was founded in 1998 by CEO J Moses.
Construction information service provider BidClerk Inc. has dropped its lawsuit against Reed Business Information and its Reed Construction Data unit. On September 29, BidClerk filed a complaint in Minnesota U.S. District Court alleging that a series of “denial of services” attacks were directed against its online system from an IP address belonging to RBI, flooding it with “millions of page views.” It also alleged that a “click fraud’” scheme aimed at BidClerk’s paid advertisements generated “hundreds of thousands” of invalid clicks and impressions on its ads.As a result of the filing, RBI says it conducted an investigation of its computer and determined that activity affecting BidClerk’s computers was not the result of intentional acts by RBI. According to RBI, BidClerk is “satisfied” with RBI’s findings and it has dropped its claim, “without any payment from RBI to settle the case.”Meanwhile, RCD’s corporate espionage complaint against McGraw-Hill Construction is ongoing.
Call 550-8422 (Anchorage) or 1-800-478-8255 (statewide) during the live broadcastPost your comment before, during or after the live broadcast (comments may be read on air).Send email to email@example.com (comments may be read on air)LIVE Broadcast: Tuesday, June 12, 2018 at 10:00 a.m. on APRN stations statewide.SUBSCRIBE: Get Talk of Alaska updates automatically by email, RSS or podcast. The Office of Children’s Services Building in Bethel. (Photo by Lakeidra Chavis/KYUK)Working with families in crisis is stressful and social workers have a high burn out rate. A new program within the Office of Children’s Services aims to help OCS field workers by providing mentors who can help them learn the skills they’ll need to not only survive, but thrive in a challenging occupation. How will that ultimately help children and families?LISTEN HEREHOST: Lori TownsendGUESTS:Kim Guay – Child Welfare Administrator, OCSKarly Shoenhair, OCS Mentor
By J. K. Schmid, Special to the AFROA $15 minimum wage bill left Maryland’s general assembly conference committee Tuesday.The bill is a reconciliation of measures that passed both houses by veto-proof margins this session.The senate bill passed 32-15.Sen. Cory V. McCray, a Democrat representing Baltimore City, cast the last vote, a vote in the bill’s favor.“I feel pretty good about it in reference to all the working folks back home in the city of Baltimore,” McCray told the AFRO. “And I know that it’ll help working families across the state of Maryland, so I’m pretty excited.”The bill appears to enjoy wide support across the state from the grass roots to leadership at the county and Baltimore City level.“It’s important to acknowledge that the Howard County Executive Calvin Ball was supportive of this legislative initiative,” McCray said. “Baltimore County’s John Olzewski, Angela Alsobrooks of Prince George’s, Marc Elrich in Montgomery County, but also Madame Mayor, who also came down and gave testimony about Senate Bill 280.”Baltimore Mayor Catherine E. Pugh joins the list of county executives after vetoing a $15 minimum wage bill March 2017.McCray’s ideal bill would seek to secure adequate funding for Maryland’s Developmental Disability Administration hiring programs and demand more exacting verification that tipped workers will earn the mandated minimum wage as it increases (an amendment proposed by Brian Feldman) over the upcoming years.Maryland’s minimum wage currently rests at $10.10 per hour. Both bills call for a general raise in the minimum wage to $15 per hour by January of 2025. The senate bill allowed for exemptions for Maryland’s smallest businesses, giving businesses with fewer than 15 employees until 2028 to raise their minimum wage.“That’s the one amendment that gave me pause, and may get worked out in conference committee,” McCray said. “The year that we’re currently in is 2019, they wouldn’t reach the minimum wage until 2028. So I’m not sure if you would agree, but that’s a long time.”Like many bills it’s a compromise, particularly over when Maryland workers will get a raise in line with Maryland’s ranking as one of the wealthiest states in the nation.“We do understand that businesses have to make adjustments and there is a need to phase the bill in over time,” Ricarra Jones told the AFRO. “Of course, we wish that it would have been sooner, but we are glad that a few years from now, working families will receive much-needed, very long overdue raises.”Jones is a longtime advocate and political organizer for the “Fight for 15.”“It’s going to take them a few extra years than we hoped for, but nevertheless we’re excited that it’s going to be happening and we still have a big hurdle to overcome, with the governor possibly vetoing the legislation when it hits his desk,” Jones said.Maryland’s legislature is only in session for a few more weeks, and Baltimore’s minimum wage bill veto wasn’t overridden due to defections and no-shows and scheduling that allowed the bill to expire.“We’re just hoping that what we’ve been doing is going to continue to work,” Jones said. “Like having these conversations, having actual workers call elected officials and saying ‘hey, I need you to stay strong on this,’ and ‘thank you for your support on this initially, but we have another hurdle and we need you continue to vote for this.’”Hogan has not committed to veto the bill; it could pass with or without his signature.So we’re not planning to switch up our efforts, things have been working so far. Just the pressure from making calls is the most powerful way to get the attention of elected leaders,” Jones said.
How AMC’s ‘The Terror’ Uses American History to Delive…33 Killed in Suspected Arson Attack on Kyoto Animation Studio in Japan KFC may be known for the Double Down sandwich, which replaced bread with two sandwich buns, but Wendy’s Japan is going one further and introducing the Wild Rock hamburger. It’s an outlandish name for an even more outlandish burger, which is great for low-carb eaters, but pretty wacky otherwise. The Wild Rock is actually a burger that doffs the bread for two hamburger patties on top and bottom, sandwiching lettuce and tomato on the inside.The burger, available at Wendy’s First Kitchen in Japan, is being advertised as a low-carb choice that only contains 4.6 grams of carbs total. Now, even though it’s low in carbs, that doesn’t mean it’s particularly healthy. It might be good if you’re watching your carbs, but the fat and calories in the burger really don’t seem like a good idea for anyone trying to eat normally.via twitter.com/NatsuKazeOtokoThe Wild Rock costs about 650 yen, which equates to $5.90. There are currently no plans, it seems, to bring the burger to Western audiences, but you could just as easily buy two burgers and make this for yourself for less money using the Double Stacks on the Value Menu, but this is a much more convenient way to get the burger together if you’re in Japan at the time.This isn’t the first weird and wild Wendy’s burger from the chain in Japan, though. Wendy’s has been known for Pikachu desserts and colored buns, just like the rest of its fast food brethren. This certainly isn’t the last of the weird foods Wendy’s has touted over the years either, as depending on the success of this burger we’ll probably see more and more like this. Let’s step it up, American fast food chains! You’re really slipping here when it comes to innovation. Need to step your game up, now.Let us know what you like about Geek by taking our survey. Stay on target
PausePlay% buffered00:0000:00UnmuteMuteDisable captionsEnable captionsSettingsCaptionsDisabledQuality0SpeedNormalCaptionsGo back to previous menuQualityGo back to previous menuSpeedGo back to previous menu0.5×0.75×Normal1.25×1.5×1.75×2×Exit fullscreenEnter fullscreen Moving forward, says Bartels, the team is working to develop and integrate other methods of high-speed 3D imaging into their SHG holography platform – specifically, 3D fluorescent imaging capabilities, which is more challenging because fluorescent light lacks the coherence that we are able to exploit for high speed SHG 3D imaging. “We’re primarily interested in studying fast dynamics in live biological tissues and systems, and are working with a number of collaborators to look at various systems that display native SHG emission. We’re also expanding the capabilities of the microscope to increase axial resolution through new hologram reconstruction algorithms and modifications of the microscope design.” Their goal is to study dynamics that have been too fast to be captured by current microscopy tools and better understand that relevant biological processes. High-speed 4D reconstructions with centroid tracking. (A) 3D reconstructions of potato starch granules with a trace marking the centroid path. Yellow indicates t = 0 ms, orange indicates t = 32 ms, and red indicates t = 63 ms. (B) 3D volume reconstructions show movement in three dimensions. Yellow indicates t = 0 ms, orange indicates t = 50 ms, and red indicates t = 92 ms. (Scale bars: 10 μm.) (A video recording of the moving particles is provided in Movie S1.) Copyright © PNAS, doi:10.1073/pnas.1306856110 Holograms set for greatness Bartels tells Phys.org that while laser scanning is the standard approach to imaging with nonlinear optical contrast, since the beams must be scanned 3D image update rates are limited – and the capture of fast dynamics are restricted to 1D or 2D images, or charting a trajectory through the specimen. “A major problem is that live specimen motion provides changes to the image that distort measurements and make it impossible to track high speed behaviors. On the other hand, SHG holography is a non-scanning technique that captures 3D information in a single shot for each volume image frame.”Bartels adds that in addition to neural circuit dynamics, the scientists are interested in observing the high speed dynamics of muscle contraction and connective tissues subjected to high speed stresses, and observing the resultant strains. “Current imaging techniques lack the ability to observe the effect of such trauma on tissues, as well as to follow the subsequent strains and relaxation or damage that occurs. SHG holography will be able to capture a time sequence of images from these tissues to better understand tissue properties. Moreover,” he continues, “SHG holography opens up imaging of dynamics that were previously not observable. As biological functions are determined by 3D organization in tissues, the ability to study high speed dynamics of biological functions – or the response of biological systems and tissues to rapid external stimuli, perturbations, or trauma – will expand our understanding of the biology, and may inform our ability to treat injuries.” © 2013 Phys.org. All rights reserved. Citation: Harmonic holograms: High-speed three-dimensional imaging captures biological dynamics (2013, November 28) retrieved 18 August 2019 from https://phys.org/news/2013-11-harmonic-holograms-high-speed-three-dimensional-imaging.html Given these challenges, Bartels says that increasing imaging speed required us to understand how to optimize the signal to noise levels of the SHG hologram and balance that optimization with an experimental design that prevented holographic fringes from washing out during high speed object motion. “We developed rigorous metrics for independently estimating noise and signal levels of a hologram, and optimized image speed, sensitivity, and signal to noise levels to obtain the optimal performance from the experimental system.” Their results suggested improvements that they plan to implement in their next-generation system. Comparison of SHG holographic reconstructions with LSM 3D images. All images were taken from a 50-μm thick slice of mouse skeletal muscle. (A) Holographic reconstruction shows connective tissue and sarcomere structures. (B) LSM 3D image of the same tissue region. (C) Volumetric overlay of the 3D images in A and B. (Scale bars: 10 μm.) Another volume reconstruction comparison is provided in SI Methods, section 13, Fig. S7. Copyright © PNAS, doi:10.1073/pnas.1306856110 Schematics of the SHG holographic microscope. (A) Off-axis holography setup is configured as a modified Mach–Zehnder interferometer. A detailed description is provided in Methods. (B) Representation of a sample emitting SHG, which is combined with the reference on a high-speed camera. (C) Holograms are numerically reconstructed, forming video-rate 3D information from a 2D hologram image. Copyright © PNAS, doi:10.1073/pnas.1306856110 “Beyond biological applications,” Bartels concludes, “I can imagine high throughput imaging of starch content for studying or monitoring biofuel production. Alternatively, kinetics of crystal formation and growth for some classes of crystals, including protein crystals, could be monitored.” (Phys.org) —In the world of biomedical science, optical microscopy rules – and nonlinear optical microscopy, which uses ultrashort pulse lasers as the illumination source, allows researchers to glean much greater detail from biological specimens. That being said, the technique’s weak signal levels significantly limit 3D image acquisition rates. Recently, however, scientists at Colorado State University employed 3D second harmonic generation achieved frame rates over 8,000 times faster than is possible with current nonlinear optical microscopy. (In second harmonic generation, or SHG, photons interact with a nonlinear material to form new photons with twice the energy and, therefore, twice the frequency and half the wavelength. While conventional optical microscopes obtain contrast by detecting variations in optical density, path length, or refractive index of the specimen, a second harmonic imaging microscope derives contrast from variations in a specimen’s ability to generate second harmonic light from incident laser light.) Moreover, the scientists introduced new methods that greatly improve the ability to quantify signal-to-noise quality. The researchers state that their study allows nonlinear optical imaging to study behavior that current experimental methodologies are unable to capture, such as neural circuit dynamics. 3D reconstructions of 350-μm thick mouse skeletal muscle at depths up to 150 μm. With the focus on the surface of the tissue as indicated in the cartoon, an SHG hologram is recorded (A) and reconstructed 75 μm deep into the tissue (B), 100 μm deep into the tissue (C), and 150 μm deep into the tissue (D). Holograms were recorded with an exposure time of 1 ms (A–C) or 4 ms (D). (Scale bars: 10 μm.) Copyright © PNAS, doi:10.1073/pnas.1306856110 Journal information: Proceedings of the National Academy of Sciences Play 3D SHG video recording of a potato starch flow recorded at 1,594.6 fps. (Scale bar: 10 μm.) Copyright © PNAS, doi:10.1073/pnas.1306856110 The main challenges in observing dynamics in live tissue, Bartels says, are the same as needed to speed up imaging to 10 mm/s – namely, brighter SHG scattering (which for a given specimen can only be increased by decreasing the illumination pulse duration) and optimizing the detection geometry. “While in this study we used a bulk ytterbium (Yb) solid state laser built in my laboratory that makes 350 femtoseconds laser pulses, we’re rebuilding the microscope with a new laser source that generates 80 fs pulses. This should increase our signals and top-end image field velocity by more than a factor of four. Also, it’s important to move the camera plane as close to the object as possible,” he points out, “as long as the fringes aren’t degraded by the moving objects.” The current paper provides a framework for evaluating an optimal experimental setup. Comparison of SHG holographic reconstructions with LSM 3D images. All images were taken from a 50-μm thick slice of mouse skeletal muscle. (A) Holographic reconstruction of collagen-rich connective tissue found in the skeletal muscle slice. (B) LSM 3D image of the same tissue region. (C) Volumetric overlay of the 3D images in A and B. (Scale bars: 10 μm.) Copyright © PNAS, doi:10.1073/pnas.1306856110 Explore further More information: Submillisecond second harmonic holographic imaging of biological specimens in three dimensions, PNAS November 12, 2013 vol. 110 no. 46 18391-18396, doi:10.1073/pnas.1306856110 The scientists have also been able to exploit coherent scattering of second harmonic light from an entire specimen volume. “We’ve already observed the benefits of coherent volume scattering by comparing images captured with SHG holography and more conventional laser scanning second harmonic holography,” Bartels notes. “In structures, such as sarcomere segments in muscle fibers, we observed significantly brighter SHG signals from holography as compared to laser scanning SHG microscopy.” He adds that the differences were less pronounced for more compact objects. Prof. Randy A. Bartels discussed the paper that he, David R. Smith and David G. Winters published recently in Proceedings of the National Academy of Sciences. “Our current configuration is capable of imaging continuously at essentially 10 mm/s for bright enough objects,” Bartels tells Phys.org. “In general, the main challenge in achieving this is making sure that the fringes don’t wash out for a given velocity – and we provide theory to easily calculate these conditions, such that the configuration in this paper was capable of recording holograms of objects moving at this velocity. If we consider an object moving at 10 mm/s,” he explains, “then in a 1-ms integration time (which we can easily do continuously), we need to resolve an object displacement of ~ 10 micron, which is roughly twenty times our current imaging resolution.” Although faster imaging is possible for a bright enough object with the camera plane moved farther away from the image plane, he adds, the object scatter enough second harmonic light to form a good hologram – and while for any particular object this is limited by the hyperpolarizability of constituent harmonophores (molecules or particles that produce second harmonic generation), the researchers can increase brightness with shorter illumination pulses.”We’re already using SHG to observe endogenous harmonophores,” Bartels continues, and are now studying live dynamics of a number of processes such as muscle contractions in embryonic models, and dynamics of structural tissues such as tendons, under high strain rates.” The team is also taking beginning steps to image action potentials of neurons. 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Kevin Durant had 29 in the Warriors Game 5 loss to the Rockets, but struggled with an inefficient 8-22 from the field.During a break in the second half action, Kerr attempted to encourage Durant to get his teammates more involved by sharing a story from his playing days about Michael Jordan heeding the advice of Phil Jackson to trust his teammates.Apparently the story din’t do the trick because the Warriors lost the game, 98-94, and Durant finished with zero assists and went scoreless in the fourth quarter. They’ll have to win Game 6 to avoid elimination. Cool story, though.
MILAN – Italian news agency ANSA says new excavations in the ancient buried city of Pompeii have yielded the undisturbed skeletons of people who took refuge from the eruption of Mount Vesuvius in A.D.79.The director of the Pompeii archaeological site, Massimo Osanna, told ANSA on Wednesday the skeletons were still intact, having been left undisturbed despite looting at the site centuries ago.Osanna called it “a shocking find, but also very important for history.”The bones — believed to be those of two women and three children — were discovered inside a house holding a charcoal inscription that historians say dates the deadly eruption to October, two months later than previously thought.Archaeologists think the people sought safety in a small room but were either crushed when the roof caved in or burned. An archaeologist inspects skeletons in the Pompeii archaeological site, Italy, Wednesday, Oct. 24, 2018. The Italian news agency ANSA says new excavations in the ancient buried city of Pompeii have yielded the undisturbed skeletons of people who had taken refuge from the eruption of Mount Vesuvius in A.D.79. The director of the Pompeii archaeological site, Massimo Osanna, told ANSA on Wednesday the skeletons, believed to be two women and three children, were still intact, having been left undisturbed despite looting at the site centuries ago. (Ciro Fusco/ANSA via AP) by The Associated Press Posted Oct 24, 2018 10:49 am PDT Last Updated Oct 24, 2018 at 11:21 am PDT AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to RedditRedditShare to 電子郵件Email Dig at Italy’s Pompeii volcanic site yields 5 skeletons
BANK OF Cyprus (BoC) does not want to foreclose properties, its chairman said on Friday, urging people who were deliberately not paying because they expected the lender to close to start meeting their obligations.Christis Hasapis said the bank was trying to work out ways with its customers so that they could pay off their loans.“Some customers who decided not to repay their loans for strategic reasons should start paying,” Hasapis said.Asked to clarify, the BoC chairman said some people thought the bank would close down if they did not pay their loans.This will not happen, Hasapis said, and those people should start paying up.“The Bank of Cyprus will find ways to help customers and businesses who clearly cannot repay their loans,” Hasapis said.Last month, BoC reported a €1.8 billion net loss for the first half of the year, impacted by its forced disposal of Greek operations and rising provisions.The bank, forced to seize client deposits to recapitalise in March, was one of three Cypriot lenders forced to sell its Greek operations in March in an attempt to ring-fence the eurozone from contagion from a messy international bailout for the island.Disposal of Greek operations resulted in a loss on disposal of €1.4 billion while impairment of loans was €539 million, BoC said. You May LikeLivestlyChip And Joanna’s $18M Mansion Is Perfect, But It’s The Backyard Everyone Is Talking AboutLivestlyUndoPopularEverythingColorado Mom Adopted Two Children, Months Later She Learned Who They Really ArePopularEverythingUndoGundry MD PrebioThrive Probiotic SupplementCardiologist: This Is What Happens To Your Body When You Eat GlutenGundry MD PrebioThrive Probiotic SupplementUndo Pensioner dies after crash on Paphos-Polis roadUndoCruise passenger airlifted to Paphos hospitalUndoRemand for pair in alleged property fraud (Updated)Undoby Taboolaby Taboola
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